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dylight 594 conjugated ulex europaeus agglutinin 1 uea1  (Vector Laboratories)


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    Vector Laboratories dylight 594 conjugated ulex europaeus agglutinin 1 uea1
    Thymic epithelial cell (TEC) subpopulations in the neonatal thymus. A: Representative flow cytometry profiles of CD45 − epithelial cell adhesion molecule (EpCAM) + TECs at postnatal days 1, 3, 5, and 7. B: Total number of TECs in the thymus from the indicated time points. C: Representative flow cytometry profiles of medullary TECs [mTECs; ulex europaeus <t>agglutinin</t> <t>1</t> <t>(UEA1)</t> + Ly51 – ] and cortical TECs (cTECs; UEA1 – Ly51 + ) within the CD45 − EpCAM + TEC population from the indicated time points. D: Total number of mTECs ( left panel ) and cTECs ( right panel ) in the thymus from the indicated mice. E: Representative flow cytometry profiles of autoimmune regulator (Aire) expression in mTECs from the indicated time points. F: Frequency ( left panel ) and absolute number ( right panel ) of Aire + mTECs. At least three independent experiments were conducted using neonatal mice. Data are presented as means ± SEM ( B , D , and F ). n = 4 or 5 mice per group ( B , D , and F ). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001. Max, maximum; NS, not significant.
    Dylight 594 Conjugated Ulex Europaeus Agglutinin 1 Uea1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dylight+594+conjugated+uea1/DyLight+594+labeled+Ulex+Europaeus+Agglutinin+I+(UEA+I)/pmc13084582-34-32-39
    Average 94 stars, based on 76 article reviews
    dylight 594 conjugated ulex europaeus agglutinin 1 uea1 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Neonatal Thymic Dynamics Influence Autoimmune Pathology by Shaping the Suppressive Potential of Regulatory T Cells"

    Article Title: Neonatal Thymic Dynamics Influence Autoimmune Pathology by Shaping the Suppressive Potential of Regulatory T Cells

    Journal: The American Journal of Pathology

    doi: 10.1016/j.ajpath.2025.12.007

    Thymic epithelial cell (TEC) subpopulations in the neonatal thymus. A: Representative flow cytometry profiles of CD45 − epithelial cell adhesion molecule (EpCAM) + TECs at postnatal days 1, 3, 5, and 7. B: Total number of TECs in the thymus from the indicated time points. C: Representative flow cytometry profiles of medullary TECs [mTECs; ulex europaeus agglutinin 1 (UEA1) + Ly51 – ] and cortical TECs (cTECs; UEA1 – Ly51 + ) within the CD45 − EpCAM + TEC population from the indicated time points. D: Total number of mTECs ( left panel ) and cTECs ( right panel ) in the thymus from the indicated mice. E: Representative flow cytometry profiles of autoimmune regulator (Aire) expression in mTECs from the indicated time points. F: Frequency ( left panel ) and absolute number ( right panel ) of Aire + mTECs. At least three independent experiments were conducted using neonatal mice. Data are presented as means ± SEM ( B , D , and F ). n = 4 or 5 mice per group ( B , D , and F ). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001. Max, maximum; NS, not significant.
    Figure Legend Snippet: Thymic epithelial cell (TEC) subpopulations in the neonatal thymus. A: Representative flow cytometry profiles of CD45 − epithelial cell adhesion molecule (EpCAM) + TECs at postnatal days 1, 3, 5, and 7. B: Total number of TECs in the thymus from the indicated time points. C: Representative flow cytometry profiles of medullary TECs [mTECs; ulex europaeus agglutinin 1 (UEA1) + Ly51 – ] and cortical TECs (cTECs; UEA1 – Ly51 + ) within the CD45 − EpCAM + TEC population from the indicated time points. D: Total number of mTECs ( left panel ) and cTECs ( right panel ) in the thymus from the indicated mice. E: Representative flow cytometry profiles of autoimmune regulator (Aire) expression in mTECs from the indicated time points. F: Frequency ( left panel ) and absolute number ( right panel ) of Aire + mTECs. At least three independent experiments were conducted using neonatal mice. Data are presented as means ± SEM ( B , D , and F ). n = 4 or 5 mice per group ( B , D , and F ). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001. Max, maximum; NS, not significant.

    Techniques Used: Flow Cytometry, Expressing

    Related Articles

    Isolation:

    Article Title: Neonatal Thymic Dynamics Influence Autoimmune Pathology by Shaping the Suppressive Potential of Regulatory T Cells.
    Article Snippet: Isolated lymphocytes were stained with the following antibodies: PE-Cy7-conjugated anti-mouse CD4 (BioLegend,San Diegp CA, GK1.5) and CD304 (BioLegend, 3E12); eFluor 450-conjugated anti-mouse CD8a (eBioscience, 53-6.7); FITC-conjugated anti-mouse CD4 (BioLegend, RM4-5) and CD44 (eBioscience, IM7); PE-conjugated anti-mouse CD62L (BioLegend, MEL-14), Foxp3 (eBioscience, FJK-16s) and Helios (Biolegend, 22F6); Alexa Fluor 700-conjugated anti-mouse CD25 (eBioscience, PC61.5); APC-conjugated anti-mouse Ki-67 (BioLegend, 16A8); and APCCy7-conjugated anti-mouse CD19 (BioLegend, 6D5); PE-Cy5.5-conjugated anti-mouse Foxp3 (eBioscience, FJK-16s). .. Isolated thymic epithelial cells (TECs) were stained with the following antibodies: BV421-conjugated anti-mouse CD45 (BD Biosciences, 30-F11); PE-Cy7-conjugated anti-mouse EpCAM (BioLegend, G8.8); Alexa Fluor 647-conjugated anti-mouse Ly-51 Jo urn al Pr -pr of 6 (BioLegend, 6C3); DyLight 594-conjugated UEA1 (Vector Laboratories, Newark, CA, DL-10671); Alexa Fluor 488-conjugated anti-mouse Aire (eBioscience, 5H12). .. Intracellular staining was performed using the Foxp3/Transcription Factor Staining Buffer Set (Invitrogen) according to the manufacturer’s instructions.

    Staining:

    Article Title: Neonatal Thymic Dynamics Influence Autoimmune Pathology by Shaping the Suppressive Potential of Regulatory T Cells.
    Article Snippet: Isolated lymphocytes were stained with the following antibodies: PE-Cy7-conjugated anti-mouse CD4 (BioLegend,San Diegp CA, GK1.5) and CD304 (BioLegend, 3E12); eFluor 450-conjugated anti-mouse CD8a (eBioscience, 53-6.7); FITC-conjugated anti-mouse CD4 (BioLegend, RM4-5) and CD44 (eBioscience, IM7); PE-conjugated anti-mouse CD62L (BioLegend, MEL-14), Foxp3 (eBioscience, FJK-16s) and Helios (Biolegend, 22F6); Alexa Fluor 700-conjugated anti-mouse CD25 (eBioscience, PC61.5); APC-conjugated anti-mouse Ki-67 (BioLegend, 16A8); and APCCy7-conjugated anti-mouse CD19 (BioLegend, 6D5); PE-Cy5.5-conjugated anti-mouse Foxp3 (eBioscience, FJK-16s). .. Isolated thymic epithelial cells (TECs) were stained with the following antibodies: BV421-conjugated anti-mouse CD45 (BD Biosciences, 30-F11); PE-Cy7-conjugated anti-mouse EpCAM (BioLegend, G8.8); Alexa Fluor 647-conjugated anti-mouse Ly-51 Jo urn al Pr -pr of 6 (BioLegend, 6C3); DyLight 594-conjugated UEA1 (Vector Laboratories, Newark, CA, DL-10671); Alexa Fluor 488-conjugated anti-mouse Aire (eBioscience, 5H12). .. Intracellular staining was performed using the Foxp3/Transcription Factor Staining Buffer Set (Invitrogen) according to the manufacturer’s instructions.



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    dylight 594 conjugated ulex europaeus agglutinin 1 uea1  (Vector Laboratories)
    94
    Vector Laboratories dylight 594 conjugated ulex europaeus agglutinin 1 uea1
    Thymic epithelial cell (TEC) subpopulations in the neonatal thymus. A: Representative flow cytometry profiles of CD45 − epithelial cell adhesion molecule (EpCAM) + TECs at postnatal days 1, 3, 5, and 7. B: Total number of TECs in the thymus from the indicated time points. C: Representative flow cytometry profiles of medullary TECs [mTECs; ulex europaeus <t>agglutinin</t> <t>1</t> <t>(UEA1)</t> + Ly51 – ] and cortical TECs (cTECs; UEA1 – Ly51 + ) within the CD45 − EpCAM + TEC population from the indicated time points. D: Total number of mTECs ( left panel ) and cTECs ( right panel ) in the thymus from the indicated mice. E: Representative flow cytometry profiles of autoimmune regulator (Aire) expression in mTECs from the indicated time points. F: Frequency ( left panel ) and absolute number ( right panel ) of Aire + mTECs. At least three independent experiments were conducted using neonatal mice. Data are presented as means ± SEM ( B , D , and F ). n = 4 or 5 mice per group ( B , D , and F ). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001. Max, maximum; NS, not significant.
    Dylight 594 Conjugated Ulex Europaeus Agglutinin 1 Uea1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dylight+594+conjugated+uea1/DyLight+594+labeled+Ulex+Europaeus+Agglutinin+I+(UEA+I)/pmc13084582-34-32-39
    Average 94 stars, based on 1 article reviews
    dylight 594 conjugated ulex europaeus agglutinin 1 uea1 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    dylight 594 conjugated uea1  (Vector Laboratories)
    94
    Vector Laboratories dylight 594 conjugated uea1
    Thymic epithelial cell (TEC) subpopulations in the neonatal thymus. A: Representative flow cytometry profiles of CD45 − epithelial cell adhesion molecule (EpCAM) + TECs at postnatal days 1, 3, 5, and 7. B: Total number of TECs in the thymus from the indicated time points. C: Representative flow cytometry profiles of medullary TECs [mTECs; ulex europaeus <t>agglutinin</t> <t>1</t> <t>(UEA1)</t> + Ly51 – ] and cortical TECs (cTECs; UEA1 – Ly51 + ) within the CD45 − EpCAM + TEC population from the indicated time points. D: Total number of mTECs ( left panel ) and cTECs ( right panel ) in the thymus from the indicated mice. E: Representative flow cytometry profiles of autoimmune regulator (Aire) expression in mTECs from the indicated time points. F: Frequency ( left panel ) and absolute number ( right panel ) of Aire + mTECs. At least three independent experiments were conducted using neonatal mice. Data are presented as means ± SEM ( B , D , and F ). n = 4 or 5 mice per group ( B , D , and F ). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001. Max, maximum; NS, not significant.
    Dylight 594 Conjugated Uea1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dylight+594+conjugated+uea1/DyLight+594+labeled+Ulex+Europaeus+Agglutinin+I+(UEA+I)/pm41485548-73-36-39
    Average 94 stars, based on 1 article reviews
    dylight 594 conjugated uea1 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Thymic epithelial cell (TEC) subpopulations in the neonatal thymus. A: Representative flow cytometry profiles of CD45 − epithelial cell adhesion molecule (EpCAM) + TECs at postnatal days 1, 3, 5, and 7. B: Total number of TECs in the thymus from the indicated time points. C: Representative flow cytometry profiles of medullary TECs [mTECs; ulex europaeus agglutinin 1 (UEA1) + Ly51 – ] and cortical TECs (cTECs; UEA1 – Ly51 + ) within the CD45 − EpCAM + TEC population from the indicated time points. D: Total number of mTECs ( left panel ) and cTECs ( right panel ) in the thymus from the indicated mice. E: Representative flow cytometry profiles of autoimmune regulator (Aire) expression in mTECs from the indicated time points. F: Frequency ( left panel ) and absolute number ( right panel ) of Aire + mTECs. At least three independent experiments were conducted using neonatal mice. Data are presented as means ± SEM ( B , D , and F ). n = 4 or 5 mice per group ( B , D , and F ). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001. Max, maximum; NS, not significant.

    Journal: The American Journal of Pathology

    Article Title: Neonatal Thymic Dynamics Influence Autoimmune Pathology by Shaping the Suppressive Potential of Regulatory T Cells

    doi: 10.1016/j.ajpath.2025.12.007

    Figure Lengend Snippet: Thymic epithelial cell (TEC) subpopulations in the neonatal thymus. A: Representative flow cytometry profiles of CD45 − epithelial cell adhesion molecule (EpCAM) + TECs at postnatal days 1, 3, 5, and 7. B: Total number of TECs in the thymus from the indicated time points. C: Representative flow cytometry profiles of medullary TECs [mTECs; ulex europaeus agglutinin 1 (UEA1) + Ly51 – ] and cortical TECs (cTECs; UEA1 – Ly51 + ) within the CD45 − EpCAM + TEC population from the indicated time points. D: Total number of mTECs ( left panel ) and cTECs ( right panel ) in the thymus from the indicated mice. E: Representative flow cytometry profiles of autoimmune regulator (Aire) expression in mTECs from the indicated time points. F: Frequency ( left panel ) and absolute number ( right panel ) of Aire + mTECs. At least three independent experiments were conducted using neonatal mice. Data are presented as means ± SEM ( B , D , and F ). n = 4 or 5 mice per group ( B , D , and F ). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001. Max, maximum; NS, not significant.

    Article Snippet: Isolated TECs were stained with the following antibodies: BV421-conjugated anti-mouse CD45 (BD Biosciences, Franklin Lakes, NJ; 30-F11); phosphatidylethanolamine-Cy7–conjugated anti-mouse epithelial cell adhesion molecule (BioLegend; G8.8); Alexa Fluor 647–conjugated anti-mouse Ly-51 (BioLegend; 6C3); DyLight 594–conjugated ulex europaeus agglutinin 1 (UEA1) (Vector Laboratories, Newark, CA; DL-1067-1); and Alexa Fluor 488–conjugated anti-mouse Aire (eBioscience; 5H12).

    Techniques: Flow Cytometry, Expressing